Fluorescent/Luminescent Reagents

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Fluorescent/Luminescent Reagents

Calcium Indicators
Enzyme Substrates
Peptidase and Protease Substrates

Calcium Indicators

Molecular Imaging Products Company offers some of the most popular fluorescent calcium indicators at very high purity and competitive prices. We also have the world largest selection of coelenterazine analogs for calcium imaging and other applications.

Calcium is an important intracellular messenger ion responsible for the activation and deactivation of numerous biological events in cells. To study the role of calcium in these events, one needs to be able to quantitatively monitor its concentration. The most widely used method of Ca2+ monitoring is by the use of fluorescentCa2+ indicators. These indicators probe Ca2+ concentration via their fluorescent spectral changes upon Ca2+ binding.

FURA-2

Fluo-3

Pluronic F-127 20% solution in DMSO 1ml

Rhod-2

A-23187 Free Acid (calcimycin; or Calcium Ionophore III) 1mg

Calcium Calibration Buffer 1 kit

Indo-1

EDC (EDAC; 1-(3-dimethylaminopropyl-3-ethylcarbodiimide, hydrochloride)) 100mg

Enzyme Substrates

We offer the highest quality chromogenic, fluorogenic and bioluminescent enzyme substrates. These are useful tools in the studies of molecular biology (detection of reporter gene expression), microbiology (food, clin. & environ.) and immunology (ELISAs).

We welcome you to inquire about our bulk prices, which can be significantly lower than our regular prices.

Substrates For ß-Glucuronidase

MUG (MUGlcU; 4-MU-glcUA; 4-methylumbelliferyl-b -D-glucuronic acid dihydrate) 100mg

Red-b -D-GlcU (Magenta-b -D-GlcA; 5-bromo-6-chloro-3-indolyl-b -D-glucuronide, cyclohexylammmonium salt) 25mg

Bioluminescent Substrates

Bioluminescence is the light produced in a biochemical reaction involving the oxidation of a substrate via an enzyme. This phenomenon has been used extensively in different formats for life science research and drug discovery owing to its extremely high sensitivity and nonhazardous nature. Examples of bioluminescence applications include calcium detection in live cells or tissues, reporter gene assays, ELISA, bioluminescence resonance energy transfer (BRET) for protein interaction studies, superoxide anion detection and high throughput drug screening.

Peptidase and Protease Substrates

Molecular Imaging Products Company offers fluorogenic peptidase and protease substrates that are based on three fluorescent dyes: rhodamine 110 (R110), 7-amino-4-trifluoromethycoumarin (AFC) and 7-amino-4-methylcoumarin (AMC or MCA). The substrates are synthesized by coupling the corboxy group of an amino acid derivative or a peptide of a specific sequence to the amine groups of the dyes. Before enzymatic hydrolysis, these substrates have no or very weak fluorescence. On enzymatic hydrolysis, the dye is released, greatly increasing the fluorescence.

AMC- or MCA-based substrates

AFT-based substrates

Rhodamine 110-based substrates